Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy.

Probing Protein Conformations at the Oil - water Interface Us - ing Single - Molecule Force Spectroscopy

Submitted for the MAR08 Meeting of The American Physical Society Probing Protein Conformations at the Oil-water Interface Using Single-Molecule Force Spectroscopy AHMED TOUHAMI, MARCELA ALEXANDER, MILENA CORREDIG , JOHN DUTCHER , University of Guelph — The present work aims at a deeper understanding of the conformational changes in Beta-lactoglobulin (BLG) protein adsorbed onto the oil-in-water...

Force probing surfaces of living cells to molecular resolution.

Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. Here we review how atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to assay the receptors' functional states, binding kinetics an...

Probing protein-protein interactions by dynamic force correlation spectroscopy.

We develop a formalism for single molecule dynamic force spectroscopy to map the energy landscape of protein-protein complex (P(1)P(2)). The joint distribution P(tau(1),tau(2)) of unbinding lifetimes tau(1) and tau(2), measurable in a compression-tension cycle, which accounts for the internal relaxation dynamics of the proteins under tension, shows that the histogram of tau(1) is not Poissonian...

Probing protein-chaperone interactions with single-molecule fluorescence spectroscopy.

Detecting Molecular Fingerprints in Single Molecule Force Spectroscopy Using Pattern Recognition

Single molecule force spectroscopy has given experimental access to the mechanical properties of protein molecules. Typically, less than 1% of the experimental recordings reflect true single molecule events due to abundant surface and multiple-molecule interactions. A key issue in single molecule force spectroscopy is thus to identify the characteristic mechanical ‘‘fingerprint’’ of a specific ...